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Volume 164, Issue 3

The YscE/YscG chaperone and YscF N-terminal sequences target YscF to the type III secretion apparatus Free

Abstract

The needle structures of type III secretion (T3S) systems are formed by the secretion and polymerization of a needle subunit protein, YscF in . A subset of T3S systems employ unique heterodimeric chaperones, YscE and YscG in , to prevent the polymerization of needle subunits within the bacterial cell. We demonstrate that the YscE/YscG chaperone is also required for stable YscF expression and for secretion of YscF. Overexpression of a functional maltose-binding protein (MBP)–YscG hybrid protein stabilized cytoplasmic YscF but YscF was not secreted in the absence of YscE. Furthermore, a YscE mutant protein was identified that functioned with YscG to stabilize cytosolic YscF; however, YscF was not secreted. These findings confirm a role for the YscE/YscG chaperone in YscF secretion and suggest that YscE may have a specific role in this process. Recent studies have shown that YscF deleted of its N-terminal 15 residues is still secreted and functional, suggesting that YscF may not require an N-terminal secretion signal. However, we demonstrate that YscF contains an N-terminal secretion signal and that a functional N-terminal signal is required for YscF secretion.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): chaperone , plague , protein secretion , type III secretion and Yersinia pestis
© 2018 The Authors
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2018-03-01
2024-04-19
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The YscE/YscG chaperone and YscF N-terminal sequences target YscF to the Yersinia pestis type III secretion apparatus
Microbiology 164, 338 (2018); https://doi.org/10.1099/mic.0.000610
/content/journal/micro/10.1099/mic.0.000610
/content/journal/micro/10.1099/mic.0.000610
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